Photolysis of caged calcium in cilia induces ciliary reversal inParamecium caudatum
نویسندگان
چکیده
منابع مشابه
Photolysis of caged calcium using a low-cost flash unit.
Photolysis of caged calcium (Nitr5, Calbiochem) can be used to study calcium dependent processes such as excitation-contraction coupling and muscular mechanics. Expensive high energy light sources are routinely used for UV light exposure, but this study describes an alternative low cost xenon flash unit constructed in our laboratory. A 300 J short arc xenon flash lamp (Heimann) was mounted in a...
متن کاملFlash photolysis of caged compounds in the cilia of olfactory sensory neurons.
Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Caged compounds are molecules made physiologically inactive by a chemical cage that can be broken by a flash of ultraviolet light. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged compounds for the study of olf...
متن کاملFlash photolysis of caged compounds
Photolabile ‘caged’ compounds are biologically inert precursors of active molecules, which when irradiated with a pulse of light free the active species at its site of action (see Fig. 1). Speed is the principal advantage offered by these probes; photochemical reactions can be very fast, with release of the active species often complete within a millisecond. The method can thus be used to study...
متن کاملCalcium waves in skinned cardiac myocytes evoked by two-photon excitation photolysis of caged calcium.
In rat ventricular myocytes chemically skinned with saponin, a local rise of [Ca2+] was achieved by two-photon excitation photolysis (TPP) of the caged Ca2+ compound 1-(2-nitro-4,5-dimethoxyphenyl)-N,N,N',N'-tetrakis[(oxycarbonyl)methyl]-1,2-ethanediamine (DM-nitrophen). Confocal Ca2+ images, by use of fluo-3, were simultaneously collected. TPP of DM-nitrophen induced Ca2+ waves propagating ove...
متن کاملIonic Control of the Reversal Response of Cilia in Paramecium caudatum
The duration of ciliary reversal of Paramecium caudatum in response to changes in external ionic factors was determined with various ionic compositions of both equilibration and stimulation media. The reversal response was found to occur when calcium ions bound by an inferred cellular cation exchange system were liberated in exchange for externally applied cations other than calcium. Factors wh...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 2003
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.00219